Identification of LAG3 high affinity aptamers by HT-SELEX and Conserved Motif Accumulation (CMA).

ABSTRACT: LAG3 receptor belongs to a family of immune-checkpoints expressed in T lymphocytes and other cells of the immune system. It plays an important role as a rheostat of the immune response. Focus on this receptor as a potential therapeutic target in cancer immunotherapy has been underscored after the success of other immune-checkpoint blockade strategies in clinical trials. LAG3 showcases the interest in the field of autoimmunity as several studies show that LAG3-targeting antibodies can also be used for the treatment of autoimmune diseases. In this work we describe the identification of a high-affinity LAG3 aptamer by High Throughput Sequencing SELEX in combination with a study of potential conserved binding modes according to sequence conservation by using 2D-structure prediction and 3D-RNA

SUBMITTER: Soldevilla MM 

PROVIDER: S-EPMC5608357 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature