Origins of the enhanced affinity of RNA-protein interactions triggered by RNA phosphorodithioate backbone modification.

ABSTRACT: The well-characterized interaction between the MS2 coat protein and its cognate RNA hairpin was used to evaluate changes in affinity as a result of phosphorodithioate (PS2) replacing phosphate by biolayer interferometry (BLI). A structure-based analysis of the data provides insights into the origins of the enhanced affinity of RNA-protein interactions triggered by the PS2 moiety.

SUBMITTER: Yang X 

PROVIDER: S-EPMC5608642 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature