Integrated network analysis to explore the key genes regulated by parathyroid hormone receptor 1 in osteosarcoma.

ABSTRACT:

Background

As an invasive malignant tumor, osteosarcoma (OS) has high mortality. Parathyroid hormone receptor 1 (PTHR1) contributes to maintaining proliferation and undifferentiated state of OS. This study is designed to reveal the action mechanisms of PTHR1 in OS.

Methods

Microarray dataset GSE46861, which included six PTHR1 knockdown OS samples and six control OS samples, was obtained from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) were identified and then performed with enrichment analysis separately using the limma package and DAVID online tool. Then, protein-protein interaction (PPI) network and module analyses were conducted using Cytoscape software. Using the WebGestalt tool, microRNAs (miRNAs) were predicted for the DEGs involved

SUBMITTER: Guan D 

PROVIDER: S-EPMC5609044 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature