Evaluation of ddRADseq for reduced representation metagenome sequencing.

ABSTRACT:

Background

Profiling of microbial communities via metagenomic shotgun sequencing has enabled researches to gain unprecedented insight into microbial community structure and the functional roles of community members. This study describes a method and basic analysis for a metagenomic adaptation of the double digest restriction site associated DNA sequencing (ddRADseq) protocol for reduced representation metagenome profiling.

Methods

This technique takes advantage of the sequence specificity of restriction endonucleases to construct an Illumina-compatible sequencing library containing DNA fragments that are between a pair of restriction sites located within close proximity. This results in a reduced sequencing library with coverage breadth that can be tuned by size selection. W

SUBMITTER: Liu MY 

PROVIDER: S-EPMC5609526 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature