Global discovery of protein kinases and other nucleotide-binding proteins by mass spectrometry.

ABSTRACT: Nucleotide-binding proteins, such as protein kinases, ATPases and GTP-binding proteins, are among the most important families of proteins that are involved in a number of pivotal cellular processes. However, global study of the structure, function, and expression level of nucleotide-binding proteins as well as protein-nucleotide interactions can hardly be achieved with the use of conventional approaches owing to enormous diversity of the nucleotide-binding protein family. Recent advances in mass spectrometry (MS) instrumentation, coupled with a variety of nucleotide-binding protein enrichment methods, rendered MS-based proteomics a powerful tool for the comprehensive characterizations of the nucleotide-binding proteome, especially the kinome. Here, we review the recent developments in the

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC5609854 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature