Association between circulating miRNAs and spinal involvement in patients with axial spondyloarthritis.

ABSTRACT:

Objectives

Dysregulation of miRNAs and their target genes contributes to the pathophysiology of autoimmune diseases. Circulating miRNAs may serve as diagnostic/prognostic biomarkers. We aimed to investigate the association between circulating miRNAs, disease activity and spinal involvement in patients with axial spondyloarthritis (AxSpA).

Methods

Total RNA was isolated from the plasma of patients with non-radiographic (nr)AxSpA, patients with ankylosing spondylitis (AS) and healthy controls (HC) via phenol-chloroform extraction. A total of 760 miRNAs were analysed with TaqMan® Low Density Arrays, and the expression of 21 miRNAs was assessed using single assays.

Results

Comprehensive analysis demonstrated the differential expression of miRNAs among patients with progre

SUBMITTER: Prajzlerova K 

PROVIDER: S-EPMC5609864 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature