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MicroRNA-199a acts as a potential suppressor of cardiomyocyte autophagy through targeting Hspa5.

ABSTRACT: Autophagy is an adaptive response to cardiomyocytes survival under stress conditions. MicroRNAs (miRNAs, miR) have been described to act as potent modulators of autophagy. To investigate whether and how miR-199a modulated autophagy in vitro, primary cardiomyocytes were treated under starvation to induce autophagy. Results showed that down-regulation of miR-199a was sufficient to activate cardiomyocytes autophagy. MiR-199a suppressed cardiomyocytes autophagy through direct inhibiting heat shock protein family A member 5 (Hspa5). Forced overexpression of Hspa5 recovered the inhibitory effect of miR-199a in autophagy activation. Our results suggested miR-199a as an effective suppressor of starvation-induced cardiomyocytes autophagy and that Hspa5 was a direct target during this process. These results extend the understanding of the role and pathway of miR-199a in cardiomyocytes autophagy, and may introduce a potential therapeutic strategy for the protection of cardiomyocytes in myocardial infarction or ischemic heart disease.

SUBMITTER: Chen L

PROVIDER: S-EPMC5609964 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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MicroRNA-199a acts as a potential suppressor of cardiomyocyte autophagy through targeting <i>Hspa5</i>.

Chen Liang L Wang Fei-Yu FY Zeng Zhen-Yu ZY Cui Ling L Shen Jian J Song Xiao-Wei XW Li Pan P Zhao Xian-Xian XX Qin Yong-Wen YW

Oncotarget 20170710 38

Autophagy is an adaptive response to cardiomyocytes survival under stress conditions. MicroRNAs (miRNAs, miR) have been described to act as potent modulators of autophagy. To investigate whether and how miR-199a modulated autophagy <i>in vitro</i>, primary cardiomyocytes were treated under starvation to induce autophagy. Results showed that down-regulation of miR-199a was sufficient to activate cardiomyocytes autophagy. MiR-199a suppressed cardiomyocytes autophagy through direct inhibiting heat ...[more]

PMID: 28969032

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Dataset Information

MicroRNA-199a acts as a potential suppressor of cardiomyocyte autophagy through targeting Hspa5.

Publications

MicroRNA-199a acts as a potential suppressor of cardiomyocyte autophagy through targeting <i>Hspa5</i>.

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