Deletion of AMPKα1 attenuates the anticontractile effect of perivascular adipose tissue (PVAT) and reduces adiponectin release.

ABSTRACT:

Background and purpose

Perivascular adipose tissue (PVAT) surrounds most blood vessels and secretes numerous active substances, including adiponectin, which produce a net anticontractile effect in healthy individuals. AMPK is a key mediator of cellular energy balance and may mediate the vascular effects of adiponectin. In this study, we investigated the role of AMPK within PVAT in mediating the anticontractile effect of PVAT.

Experimental approach

Endothelium-denuded aortic rings from wild-type (WT; Sv129) and α₁ AMPK knockout (KO) mice were mounted on a wire myograph. Dose-response curves to the AMPK-independent vasodilator cromakalim were studied in vessels with and without PVAT, and effect of pre-incubation with conditioned media and adiponectin on relaxation w

SUBMITTER: Almabrouk TAM 

PROVIDER: S-EPMC5610155 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature