Different developmental histories of beta-cells generate functional and proliferative heterogeneity during islet growth.

ABSTRACT: The proliferative and functional heterogeneity among seemingly uniform cells is a universal phenomenon. Identifying the underlying factors requires single-cell analysis of function and proliferation. Here we show that the pancreatic beta-cells in zebrafish exhibit different growth-promoting and functional properties, which in part reflect differences in the time elapsed since birth of the cells. Calcium imaging shows that the beta-cells in the embryonic islet become functional during early zebrafish development. At later stages, younger beta-cells join the islet following differentiation from post-embryonic progenitors. Notably, the older and younger beta-cells occupy different regions within the islet, which generates topological asymmetries in glucose responsiveness and proliferation. Sp

SUBMITTER: Singh SP 

PROVIDER: S-EPMC5610262 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature