RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane.

ABSTRACT: The human respiratory syncytial virus G protein plays an important role in the entry and assembly of filamentous virions. Here, we report the use of fluorescently labeled soybean agglutinin to selectively label the respiratory syncytial virus G protein in living cells without disrupting respiratory syncytial virus infectivity or filament formation and allowing for interrogations of respiratory syncytial virus virion assembly. Using this approach, we discovered that plasma membrane-bound respiratory syncytial virus G rapidly recycles from the membrane via clathrin-mediated endocytosis. This event is then followed by the dynamic formation of filamentous and branched respiratory syncytial virus particles, and assembly with genomic ribonucleoproteins and caveolae-associated vesicles prior to r

SUBMITTER: Vanover D 

PROVIDER: S-EPMC5610308 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature