Determination of extracellular matrix collagen fibril architectures and pathological remodeling by polarization dependent second harmonic microscopy.

ABSTRACT: Polarization dependence second harmonic generation (P-SHG) microscopy is gaining increase popularity for in situ quantification of fibrillar protein architectures. In this report, we combine P-SHG microscopy, new linear least square (LLS) fitting and modeling to determine and convert the complex second-order non-linear optical anisotropy parameter ρ of several collagen rich tissues into a simple geometric organization of collagen fibrils. Modeling integrates a priori knowledge of polyhelical organization of collagen molecule polymers forming fibrils and bundles of fibrils as well as Poisson photonic shot noise of the detection system. The results, which accurately predict the known sub-microscopic hierarchical organization of collagen fibrils in several tissues, suggest that they can be su

SUBMITTER: Rouede D 

PROVIDER: S-EPMC5610346 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature