^* Optimization of Preculture Conditions to Maximize the In Vivo Performance of Cell-Seeded Engineered Intervertebral Discs.

ABSTRACT: The development of engineered tissues has progressed over the past 20 years from in vitro characterization to in vivo implementation. For musculoskeletal tissue engineering in particular, the emphasis of many of these studies was to select conditions that maximized functional and compositional gains in vitro. However, the transition from the favorable in vitro culture environment to a less favorable in vivo environment has proven difficult, and, in many cases, engineered tissues do not retain their preimplantation phenotype after even short periods in vivo. Our laboratory recently developed disc-like angle-ply structures (DAPS), an engineered intervertebral disc for total disc replacement. In this study, we tested six different preculture media formulations (three serum-containing and thre

SUBMITTER: Martin JT 

PROVIDER: S-EPMC5610389 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature