Characterization of the tertiary structure of the peptidoglycan of Enterococcus faecalis.

ABSTRACT: Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of ¹³C and ¹⁵N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan. The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems. Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-¹³C]alanyl and l-[ε-¹⁵N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge arch

SUBMITTER: Yang H 

PROVIDER: S-EPMC5610627 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature