Feline leukocyte adhesion (CD18) deficiency caused by a deletion in the integrin β₂ (ITGB2) gene.

ABSTRACT:

Background

Leukocyte adhesion deficiency (LAD) or CD18 deficiency is an autosomal recessive immunodeficiency which has been described in people, cattle, dogs, and knockout mice.

Objectives

The study goals were to characterize the clinicopathologic, immunologic, and molecular genetic features of feline LAD (FLAD) in a neutered male adult Domestic Longhair cat with severe leukocytosis and recurrent infections.

Methods

Flow cytometry evaluated surface expression of CD18 on neutrophils. In vitro functional assays assessed CD18-dependent neutrophil adhesion and T-cell proliferation. Genomic DNA and cDNA were used to identify a causative mutation in the coding sequence of the integrin β₂ subunit (ITGB2) gene.

Results

The affected cat developed periodontiti

SUBMITTER: Bauer TR 

PROVIDER: S-EPMC5610637 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature