In Vivo Rescue of the Hematopoietic Niche By Pluripotent Stem Cell Complementation of Defective Osteoblast Compartments.

ABSTRACT: Bone-forming osteoblasts play critical roles in supporting bone marrow hematopoiesis. Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSC), are capable of differentiating into osteoblasts. To determine the capacity of stem cells needed to rescue aberrant skeletal development and bone marrow hematopoiesis in vivo, we used a skeletal complementation model. Mice deficient in Runx2, a master transcription factor for osteoblastogenesis, fail to form a mineralized skeleton and bone marrow. Wild-type (WT) green fluorescent protein (GFP)⁺ ESCs and yellow fluorescent protein (YFP)⁺ iPSCs were introduced into Runx2-null blastocyst-stage embryos. We assessed GFP/YFP⁺ cell contribution by whole-mount fluorescence and histological

SUBMITTER: Chubb R 

PROVIDER: S-EPMC5610643 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature