Synergistic Modulation of Inflammatory but not Metabolic Effects of High-Fat Feeding by CCR2 and CX3CR1.

ABSTRACT:

Objective

The purpose of the study was to explore the impact of dual targeting of C-C motif chemokine receptor-2 (CCR2) and fractalkine receptor (CX3CR1) on the metabolic and inflammatory consequences of obesity induced by a high-fat diet (HFD).

Methods

C57BL/6J wild-type, Cx3cr1^-/- , Ccr2^-/- , and Cx3cr1^-/- Ccr2^-/- double-knockout male and female mice were fed a 45% HFD for up to 25 weeks starting at 12 weeks of age.

Results

All groups gained weight at a similar rate and developed a similar degree of adiposity, hyperglycemia, glucose intolerance, and impairment of insulin sensitivity in response to HFD. As expected, the circulating monocyte count was decreased in Ccr2^-/- and Cx3cr1^-/- Ccr2^-/-

SUBMITTER: Zhang H 

PROVIDER: S-EPMC5610963 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature