β₁-Integrin-Mediated Adhesion Is Lipid-Bilayer Dependent.

ABSTRACT: Integrin-mediated adhesion is a central feature of cellular adhesion, locomotion, and endothelial cell mechanobiology. Although integrins are known to be transmembrane proteins, little is known about the role of membrane biophysics and dynamics in integrin adhesion. We treated human aortic endothelial cells with exogenous amphiphiles, shown previously in model membranes, and computationally, to affect bilayer thickness and lipid phase separation, and subsequently measured single-integrin-molecule adhesion kinetics using an optical trap, and diffusion using fluorescence correlation spectroscopy. Benzyl alcohol (BA) partitions to liquid-disordered (L_d) domains, thins them, and causes the greatest increase in hydrophobic mismatch between liquid-ordered (L_o) and L_d</

SUBMITTER: Son S 

PROVIDER: S-EPMC5611673 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature