Acceleration Strategies to Enhance Metabolic Ensemble Modeling Performance.

ABSTRACT: Developing reliable, predictive kinetic models of metabolism is a difficult, yet necessary, priority toward understanding and deliberately altering cellular behavior. Constraint-based modeling has enabled the fields of metabolic engineering and systems biology to make great strides in interrogating cellular metabolism but does not provide sufficient insight into regulation or kinetic limitations of metabolic pathways. Moreover, the growth-optimized assumptions that constraint-based models often rely on do not hold when studying stationary or persistor cell populations. However, developing kinetic models provides many unique challenges, as many of the kinetic parameters and rate laws governing individual enzymes are unknown. Ensemble modeling (EM) was developed to circumnavigate this challe

SUBMITTER: Greene JL 

PROVIDER: S-EPMC5611682 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature