Neuronal-expressed microRNA-targeted pseudogenes compete with coding genes in the human brain.

ABSTRACT: MicroRNAs orchestrate brain functioning via interaction with microRNA recognition elements (MRE) on target transcripts. However, the global impact of potential competition on the microRNA pool between coding and non-coding brain transcripts that share MREs with them remains unexplored. Here we report that non-coding pseudogene transcripts carrying MREs (PSG^+MRE) often show duplicated origin, evolutionary conservation and higher expression in human temporal lobe neurons than comparable duplicated MRE-deficient pseudogenes (PSG^-MRE). PSG^+MRE participate in neuronal RNA-induced silencing complexes (RISC), indicating functional involvement. Furthermore, downregulation cell culture experiments validated bidirectional co-regulation of PSG^+MRE with MRE-

SUBMITTER: Barbash S 

PROVIDER: S-EPMC5611730 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature