Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.

ABSTRACT: Branching morphogenesis creates arborized epithelial networks. In the mammalian kidney, an epithelial progenitor pool at ureteric branch tips (UBTs) creates the urine-transporting collecting system. Using region-specific mouse reporter strains, we performed an RNA-seq screen, identifying tip- and stalk-enriched gene sets in the developing collecting duct system. Detailed in situ hybridization studies of tip-enriched predictions identified UBT-enriched gene sets conserved between the mouse and human kidney. Comparative spatial analysis of their UBT niche expression highlighted distinct patterns of gene expression revealing novel molecular heterogeneity within the UBT progenitor population. To identify kidney-specific and shared programs of branching morphogenesis, comparative express

SUBMITTER: Rutledge EA 

PROVIDER: S-EPMC5611955 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature