Unraveling the interactions of the physiological reductant flavodoxin with the different conformations of the Fe protein in the nitrogenase cycle.

ABSTRACT: Nitrogenase reduces dinitrogen (N₂) to ammonia in biological nitrogen fixation. The nitrogenase Fe protein cycle involves a transient association between the reduced, MgATP-bound Fe protein and the MoFe protein and includes electron transfer, ATP hydrolysis, release of P_i, and dissociation of the oxidized, MgADP-bound Fe protein from the MoFe protein. The cycle is completed by reduction of oxidized Fe protein and nucleotide exchange. Recently, a kinetic study of the nitrogenase Fe protein cycle involving the physiological reductant flavodoxin reported a major revision of the rate-limiting step from MoFe protein and Fe protein dissociation to release of P_i Because the Fe protein cannot interact with flavodoxin and the MoFe protein simultaneously, knowledge o

SUBMITTER: Pence N 

PROVIDER: S-EPMC5612100 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature