MicroPET/CT Imaging of AXL Downregulation by HSP90 Inhibition in Triple-Negative Breast Cancer.

ABSTRACT: AXL receptor tyrosine kinase is overexpressed in a number of solid tumor types including triple-negative breast cancer (TNBC). AXL is considered an important regulator of epithelial-to-mesenchymal transition (EMT) and a potential therapeutic target for TNBC. In this work, we used microPET/CT with ⁶⁴Cu-labeled anti-human AXL antibody (⁶⁴Cu-anti-hAXL) to noninvasively interrogate the degradation of AXL in vivo in response to 17-allylamino-17-demethoxygeldanamycin (17-AAG), a potent inhibitor of HSP90. 17-AAG treatment caused significant decline in AXL expression in orthotopic TNBC MDA-MB-231 tumors, inhibited EMT, and delayed tumor growth in vivo, resulting in significant reduction in tumor uptake of ⁶⁴Cu-anti-hAXL as clearly visualized by microPET/CT. Our da

SUBMITTER: Wang W 

PROVIDER: S-EPMC5612679 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature