Adenoviral vector mediated ferritin over-expression in mesenchymal stem cells detected by 7T MRI in vitro.

ABSTRACT:

Objective

The aim of the present work was to verify whether adenoviral vector mediated ferritin over-expression in mesenchymal stem cells could be detected by 7T MRI device, and to explore the relationship between ferritin content and MRI signal intensities.

Methods

A recombined adenoviral vector (rAdV) encoding ferritin heavy chain (FTH1) subunit was specially designed for the aim of infecting bone marrow mesenchymal stem cells (BMSCs). Ferritin over-expression in BMSCs was determined by cell immunocytochemistry and the ferritin content in cells was determined by ELISA assay. BMSCs were subjected to cell viability, proliferation and multi-differentiation analyses as well as 7T MRI test using fast spin-echo pulse sequence. The R2 value andδR2 were calculated according to T2

SUBMITTER: Dai HY 

PROVIDER: S-EPMC5612726 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature