Efficacy and Safety of Pancreas-Targeted Hydrodynamic Gene Delivery in Rats.

ABSTRACT: Development of an effective, safe, and convenient method for gene delivery to the pancreas is a critical step toward gene therapy for pancreatic diseases. Therefore, we tested the possibility of applying the principle of hydrodynamic gene delivery for successful gene transfer to pancreas using rats as a model. The established procedure involves the insertion of a catheter into the superior mesenteric vein with temporary blood flow occlusion at the portal vein and hydrodynamic injection of DNA solution. We demonstrated that our procedure achieved efficient pancreas-specific gene expression that was 2,000-fold higher than that seen in the pancreas after the systemic hydrodynamic gene delivery. In addition, the level of gene expression achieved in the pancreas by the pancreas-specific gene de

SUBMITTER: Ogawa K 

PROVIDER: S-EPMC5612811 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature