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Methods to generate genetically engineered mouse models of soft tissue sarcoma.

ABSTRACT: We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-Kras(G12D/+); p53(flox/flox)). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer therapies, metastasis, in vivo imaging, and genetic requirements for tumorigenesis.

SUBMITTER: Dodd RD 

PROVIDER: S-EPMC5612825 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Methods to generate genetically engineered mouse models of soft tissue sarcoma.

Dodd Rebecca D RD   Añó Leonor L   Blum Jordan M JM   Li Zhizhong Z   Van Mater David D   Kirsch David G DG  

Methods in molecular biology (Clifton, N.J.) 20150101

We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-Kras(G12D/+); p53(flox/flox)). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer  ...[more]

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