Regional differences in genetic susceptibility to non-alcoholic liver disease in two distinct Indian ethnicities.

ABSTRACT:

Aim

To validate the association of variants in PNPLA3 (rs2281135) and TM6SF2 (rs58542926) genes with ultrasound detected non-alcoholic fatty liver disease (NAFLD).

Methods

A total of 503 individuals with and without fatty infiltration were recruited. Fatty infiltration was confirmed based on ultrasound findings. Anthropometric data and blood samples were collected from the study group. DNA was isolated from peripheral blood, quality and quantity was assessed by gel electrophoresis and spectrophotometer respectively. Genotyping of the variants in PNPLA3 and TM6SF2 genes was carried out by employing taqman probes (C_15875080_10 for PNPLA3 and C_8946351_10 for TM6SF2 SNP) on real time PCR (Stepone-Lifetechnologies). Genotype data was tested for devia

SUBMITTER: Bale G 

PROVIDER: S-EPMC5612841 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature