Insulin prevents aberrant mitochondrial phenotype in sensory neurons of type 1 diabetic rats.

ABSTRACT: Diabetic neuropathy affects approximately 50% of diabetic patients. Down-regulation of mitochondrial gene expression and function has been reported in both human tissues and in dorsal root ganglia (DRG) from animal models of type 1 and type 2 diabetes. We hypothesized that loss of direct insulin signaling in diabetes contributes to loss of mitochondrial function in DRG neurons and to development of neuropathy. Sensory neurons obtained from age-matched adult control or streptozotocin (STZ)-induced type 1 diabetic rats were cultured with or without insulin before determining mitochondrial respiration and expression of mitochondrial respiratory chain and insulin signaling-linked proteins. For in vivo studies age-matched control rats and diabetic rats with or without trace insulin supplementat

SUBMITTER: Aghanoori MR 

PROVIDER: S-EPMC5612919 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature