Inducible Expression of spo0A as a Universal Tool for Studying Sporulation in Clostridium difficile.

ABSTRACT: Clostridium difficile remains a leading nosocomial pathogen, putting considerable strain on the healthcare system. The ability to form endospores, highly resistant to environmental insults, is key to its persistence and transmission. However, important differences exist between the sporulation pathways of C. difficile and the model Gram-positive organism Bacillus subtilis. Amongst the challenges in studying sporulation in C. difficile is the relatively poor levels of sporulation and high heterogeneity in the sporulation process. To overcome these limitations we placed P_tet regulatory elements upstream of the master regulator of sporulation, spo0A, generating a new strain that can be artificially induced to sporulate by addition of anhydr

SUBMITTER: Dembek M 

PROVIDER: S-EPMC5613124 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature