The EspF N-Terminal of Enterohemorrhagic Escherichia coli O157:H7 EDL933w Imparts Stronger Toxicity Effects on HT-29 Cells than the C-Terminal.

ABSTRACT: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 EspF is an important multifunctional protein that destroys the tight junctions of intestinal epithelial cells and promotes host cell apoptosis. However, its molecular mechanism remains elusive. We knocked out the espF sequence (747 bp, ΔespF), N-terminal sequence (219 bp, ΔespF_N ), and C-terminal sequence (528 bp, ΔespF_C ) separately using the pKD46-mediated λ Red homologous recombination system. Then, we built the corresponding complementation strains, namely, ΔespF/pespF, ΔespF_N/pespF_N , and ΔespF_C/pespF_C by overlap PCR, which were used in infecting HT-29 cells an

SUBMITTER: Wang X 

PROVIDER: S-EPMC5613159 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature