Pre-storage centrifugation conditions have significant impact on measured microRNA levels in biobanked EDTA plasma samples.

ABSTRACT:

Background

In the past few years, an increasing number of studies have reported the potential use of microRNAs (miRNA) as circulating biomarkers for diagnosis or prognosis of a wide variety of diseases. There is, however, a lack of reproducibility between studies. Due to the high miRNA content in platelets this may partly be explained by residual platelets in the plasma samples used. When collecting fresh plasma samples, it is possible to produce cell-free/platelet-poor plasma by centrifugation. In this study, we systematically investigated whether biobanked EDTA plasma samples could be processed to be suitable for miRNA analysis.

Materials and methods

Blood samples were collected from ten healthy volunteers and centrifuged to produce platelet-poor-plasma (PPP) and standard

SUBMITTER: Binderup HG 

PROVIDER: S-EPMC5613297 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature