Ontology highlight
ABSTRACT:
SUBMITTER: Escos M
PROVIDER: S-EPMC5613351 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Biochemistry and biophysics reports 20160608
The cytosolic form of phosphoenolpyruvate carboxykinase (PCK1) plays a regulatory role in gluconeogenesis and glyceroneogenesis. The role of the mitochondrial isoform (PCK2) remains unclear. We report the partial purification and kinetic and functional characterization of human PCK2. Kinetic properties of the enzyme are very similar to those of the cytosolic enzyme. PCK2 has an absolute requirement for Mn<sup>2+</sup> ions for activity; Mg<sup>2+</sup> ions reduce the K<sub>m</sub> for Mn<sup>2+ ...[more]