Contribution of single amino acid and codon substitutions to the production and secretion of a lipase by Bacillus subtilis.

ABSTRACT:

Background

Bacillus subtilis produces and secretes proteins in amounts of up to 20 g/l under optimal conditions. However, protein production can be challenging if transcription and cotranslational secretion are negatively affected, or the target protein is degraded by extracellular proteases. This study aims at elucidating the influence of a target protein on its own production by a systematic mutational analysis of the homologous B. subtilis model protein lipase A (LipA). We have covered the full natural diversity of single amino acid substitutions at 155 positions of LipA by site saturation mutagenesis excluding only highly conserved residues and qualitatively and quantitatively screened about 30,000 clones for extracellular LipA production. Identified variants with beneficial ef

SUBMITTER: Skoczinski P 

PROVIDER: S-EPMC5613506 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature