Resveratrol inhibits plasma membrane Ca²⁺-ATPase inducing an increase in cytoplasmic calcium.

ABSTRACT: Plasma membrane Ca²⁺-ATPase (PMCA) plays a vital role in maintaining cytosolic calcium concentration ([Ca²⁺] _i ). Given that many diseases have modified PMCA expression and activity, PMCA is an important potential target for therapeutic treatment. This study demonstrates that the non-toxic, naturally-occurring polyphenol resveratrol (RES) induces increases in [Ca²⁺] _i via PMCA inhibition in primary dermal fibroblasts and MDA-MB-231 breast cancer cells. Our results also illustrate that RES and the fluorescent intracellular calcium indicator Fura-2, are compatible for simultaneous use, in contrast to previous studies, which indicated that RES modulates the Fura-2 fluorescence independent of calcium concentration. Because RE

SUBMITTER: Peterson JA 

PROVIDER: S-EPMC5613515 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature