Three-dimensional image reconstruction of distribution of Pnmt⁺ cell-derived cells in murine heart.

ABSTRACT: Elucidating the function of specific cell types in a highly complex multicellular system such as the heart often requires detailed anatomic reconstruction. We recently described a distinctive class of phenylethanolamine n-methyltransferase (Pnmt⁺) cell-derived cardiomyocytes (PdCMs), a new cardiomyocyte population with a potential endocrine role. In this dataset, a 3D reconstruction was carried out to visualise the distribution of PdCMs throughout the murine heart. Rigid registration (stiff rotation and translation) was applied to properly align the fused heart slice images based on landmarks using TrakEM2, an open source plug-in in Fiji. The registered slices were then analysed and reconstructed using MATLAB (MATLAB^®. Version 8.3.0.532). The final reconstructed 3D vo

SUBMITTER: Ni H 

PROVIDER: S-EPMC5613735 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature