Paced monophasic and biphasic waveforms alter transmembrane potentials and metabolism of human fibroblasts.

ABSTRACT: Resting transmembrane potential (TMP) of primary human fibroblast cells was altered in predictable directions by subjecting cell cultures to specific monophasic and biphasic waveforms. Cells electrically stimulated with an anodal pulse resulted in hyperpolarization while a cathodal waveform depolarized the TMP to below that of non-paced control cells. The biphasic waveform, consisting of an anodal pulse followed immediately by an inverse symmetric cathodal pulse, also lessened the TMP similar to that of the cathodal pulse. The effect of short-term pacing on the TMP can last up to 4 h before the potentials equilibrate back to baseline. While subjecting the cells to this electrical field stimulation did not appear to damage the integrity of the cells, the three paced electrical stimulation w

SUBMITTER: O'Hearn SF 

PROVIDER: S-EPMC5613960 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature