Abundance of RNase4 and RNase5 mRNA and protein in host defence related tissues and secretions in cattle.

ABSTRACT: Members of the RNaseA family are present in various tissues and secretions but their function is not well understood. Some of the RNases are proposed to participate in host defence. RNase4 and RNase5 are present in cows' milk and have antimicrobial activity. However, their presence in many tissues and secretions has not been characterised. We hypothesised that these two RNases are present in a range of tissues and secretions where they could contribute to host defence. We therefore, determined the relative abundance of RNase4 and RNase5 mRNA as well as protein levels in a range of host defence related and other tissues as well as a range of secretions in cattle, using real time PCR and western blotting. The two RNases were found to be expressed in liver, lung, pancreas, mammary gland, plac

SUBMITTER: Gupta SK 

PROVIDER: S-EPMC5613968 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature