Thermal stability of pepsin: A predictive thermodynamic model of a multi-domain protein.

ABSTRACT: Pepsin is generally used in the preparation of F(ab)₂ fragments from antibodies. The antibodies that are one of the largest and fastest growing categories of bio- pharmaceutical candidates. Differential scanning calorimetric is principally suitable method to follow the energetics of a multi-domain, fragment to perform a more exhaustive description of the thermodynamics in an associating system. The thermodynamical models of analysis include the construction of a simultaneous fitting of a theoretical expression. The expression depending on the equilibrium unfolding data from multimeric proteins that have a two-state monomer. The aim of the present study is considering the DSC data in connection with pepsin going through reversible thermal denaturation. Afterwards, we calculate th

SUBMITTER: Rastegari AA 

PROVIDER: S-EPMC5614592 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature