Generation of an immortalized mesenchymal stem cell line producing a secreted biosensor protein for glucose monitoring.

ABSTRACT: Diabetes is a chronic disease characterized by high levels of blood glucose. Diabetic patients should normalize these levels in order to avoid short and long term clinical complications. Presently, blood glucose monitoring is dependent on frequent finger pricking and enzyme based systems that analyze the drawn blood. Continuous blood glucose monitors are already on market but suffer from technical problems, inaccuracy and short operation time. A novel approach for continuous glucose monitoring is the development of implantable cell-based biosensors that emit light signals corresponding to glucose concentrations. Such devices use genetically modified cells expressing chimeric genes with glucose binding properties. MSCs are good candidates as carrier cells, as they can be genetically enginee

SUBMITTER: Siska EK 

PROVIDER: S-EPMC5614622 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature