Exosome-mediated miR-146a transfer suppresses type I interferon response and facilitates EV71 infection.

ABSTRACT: Exosomes can transfer genetic materials between cells. Their roles in viral infections are beginning to be appreciated. Researches have shown that exosomes released from virus-infected cells contain a variety of viral and host cellular factors that are able to modulate recipient's cellular response and result in productive infection of the recipient host. Here, we showed that EV71 infection resulted in upregulated exosome secretion and differential packaging of the viral genomic RNA and miR-146a into exosomes. We provided evidence showing that miR-146a was preferentially enriched in exosomes while the viral RNA was not in infected cells. Moreover, the exosomes contained replication-competent EV71 RNA in complex with miR-146a, Ago2, and GW182 and could mediate EV71 transmission independent

SUBMITTER: Fu Y 

PROVIDER: S-EPMC5614653 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature