Peptide oligomers from ultra-short peptides using sortase.

ABSTRACT: Sortase A catalyzed ligation of ultra-short peptides leads to inter/intra-molecular transpeptidation to form either linear or cyclic oligomers dependent upon the peptide length. Cyclic peptides were the main products for peptides with more than 15aa. However, for ultra-short (<15aa) peptides, cyclic oligomers became predominant in prolonged reactions. Peptides with 1-3 aminoglycines were equally active but peptide oligomers from peptide containing more than one aminoglycine were prone to hydrolysis.

SUBMITTER: Voloshchuk N 

PROVIDER: S-EPMC5614665 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature