Nitric oxide inhibits ATPase activity and induces resistance to topoisomerase II-poisons in human MCF-7 breast tumor cells.

ABSTRACT:

Background

Topoisomerase poisons are important drugs for the management of human malignancies. Nitric oxide (^•NO), a physiological signaling molecule, induces nitrosylation (or nitrosation) of many cellular proteins containing cysteine thiol groups, altering their cellular functions. Topoisomerases contain several thiol groups which are important for their activity and are also targets for nitrosation by nitric oxide.

Methods

Here, we have evaluated the roles of ^• NO/ ^• NO-derived species in the stability and activity of topo II (α and β) both in vitro and in human MCF-7 breast tumor cells. Furthermore, we have examined the effects of ^• NO on the ATPase activity of topo II.

Results

Treatment of purified

SUBMITTER: Sinha BK 

PROVIDER: S-EPMC5614683 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature