The delayed effect of mustard gas on housekeeping gene expression in lung biopsy of chemical injuries.

ABSTRACT:

Objective

Sulfur mustard (SM) was used as a chemical weapon in Iraq-Iran war. Exposed people have major complications in important organs such as pulmonary system. Some studies have shown that SM could affect the expression of endogenous genes and non-housekeeping genes, time dependently. To understand the accurate molecular mechanism of the delayed effect of SM, the identification of the gene expression pattern in these patients is essential. Hence, we have evaluated mRNA expression of four common housekeeping genes (ACTIN, PGK1, β2m, GAPDH) in SM-exposed and non-exposed (control) formalin-fixed, paraffin-embedded (FFPE) human lung tissues.

Method

Paraffin block of lung biopsy of SM-exposed people (11 cases) and people without exposure to SM as control group (9 cases) have

SUBMITTER: Eghtedardoost M 

PROVIDER: S-EPMC5614689 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature