Ontology highlight
ABSTRACT:
SUBMITTER: Chow RD
PROVIDER: S-EPMC5614841 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Nature neuroscience 20170814 10
A causative understanding of genetic factors that regulate glioblastoma pathogenesis is of central importance. Here we developed an adeno-associated virus-mediated, autochthonous genetic CRISPR screen in glioblastoma. Stereotaxic delivery of a virus library targeting genes commonly mutated in human cancers into the brains of conditional-Cas9 mice resulted in tumors that recapitulate human glioblastoma. Capture sequencing revealed diverse mutational profiles across tumors. The mutation frequencie ...[more]