Adaptation trajectories during adhesion and spreading affect future cell states.

ABSTRACT: Cells are complex systems in which dynamic gene expression and protein-interaction networks adapt to changes in the environment. Seeding and subsequent spreading of cells on substrates represents an example of adaptation to a major perturbation. The formation of adhesive interactions and self-organisation of the cytoskeleton during initial spreading might prime future cell behaviour. To elucidate the role of these events on later cellular behaviour, we mapped the trajectories by which cells respond to seeding on substrates with different physical properties. Our experiments on cell spreading dynamics on collagen- or fibrin-coated polyacrylamide gels and collagen or fibrin hydrogels show that on each substrate, cells follow distinct trajectories of morphological changes, culminating in fund

SUBMITTER: Bruekers SMC 

PROVIDER: S-EPMC5615062 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature