Low-dose dose-response for reduced cell viability after exposure of human keratinocyte (HEK001) cells to arsenite.

ABSTRACT: The in vitro arsenite (As^III) cytotoxicity dose-response (DR) of human keratinocytes (HEK001) was examined at greater statistical resolution than ever previously reported using the MTT assay to determine cell viability. Fifty-four 96-well plates were treated with As^III concentrations of 0.25, 0.5, 1, 2, 3, 4, 5, 7, 10, 15, 20, 25, or 30 μM. Because of unexpected variation in viability response patterns, a two-stage DR analysis was used in which data on plate-specific viability (%), estimated as 100% times the ratio of measured viability in exposed to unexposed cells, were fit initially to a generalized lognormal response function positing that HEK001 cells studied consisted of: a proportion P of relatively highly sensitive (HS) cells, a proportion P</

SUBMITTER: Bogen KT 

PROVIDER: S-EPMC5615095 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature