Process development of human multipotent stromal cell microcarrier culture using an automated high-throughput microbioreactor.

ABSTRACT: Microbioreactors play a critical role in process development as they reduce reagent requirements and can facilitate high-throughput screening of process parameters and culture conditions. Here, we have demonstrated and explained in detail, for the first time, the amenability of the automated ambr15 cell culture microbioreactor system for the development of scalable adherent human mesenchymal multipotent stromal/stem cell (hMSC) microcarrier culture processes. This was achieved by first improving suspension and mixing of the microcarriers and then improving cell attachment thereby reducing the initial growth lag phase. The latter was achieved by using only 50% of the final working volume of medium for the first 24 h and using an intermittent agitation strategy. These changes resulted in >15

SUBMITTER: Rafiq QA 

PROVIDER: S-EPMC5615370 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature