Accelerating bacterial growth detection and antimicrobial susceptibility assessment in integrated picoliter droplet platform.

ABSTRACT: There remains an urgent need for rapid diagnostic methods that can evaluate antibiotic resistance for pathogenic bacteria in order to deliver targeted antibiotic treatments. Toward this end, we present a rapid and integrated single-cell biosensing platform, termed dropFAST, for bacterial growth detection and antimicrobial susceptibility assessment. DropFAST utilizes a rapid resazurin-based fluorescent growth assay coupled with stochastic confinement of bacteria in 20 pL droplets to detect signal from growing bacteria after 1h incubation, equivalent to 2-3 bacterial replications. Full integration of droplet generation, incubation, and detection into a single, uninterrupted stream also renders this platform uniquely suitable for in-line bacterial phenotypic growth assessment. To illustrate t

SUBMITTER: Kaushik AM 

PROVIDER: S-EPMC5615843 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature