A Spatial Interactome Reveals the Protein Organization of the Algal CO₂-Concentrating Mechanism.

ABSTRACT: Approximately one-third of global CO₂ fixation is performed by eukaryotic algae. Nearly all algae enhance their carbon assimilation by operating a CO₂-concentrating mechanism (CCM) built around an organelle called the pyrenoid, whose protein composition is largely unknown. Here, we developed tools in the model alga Chlamydomonas reinhardtii to determine the localizations of 135 candidate CCM proteins and physical interactors of 38 of these proteins. Our data reveal the identity of 89 pyrenoid proteins, including Rubisco-interacting proteins, photosystem I assembly factor candidates, and inorganic carbon flux components. We identify three previously undescribed protein layers of the pyrenoid: a plate-like layer, a mesh layer, and a punctate layer. We find that the carb

SUBMITTER: Mackinder LCM 

PROVIDER: S-EPMC5616186 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature