Influence of node abundance on signaling network state and dynamics analyzed by mass cytometry.

ABSTRACT: Signaling networks are key regulators of cellular function. Although the concentrations of signaling proteins are perturbed in disease states, such as cancer, and are modulated by drug therapies, our understanding of how such changes shape the properties of signaling networks is limited. Here we couple mass-cytometry-based single-cell analysis with overexpression of tagged signaling proteins to study the dependence of signaling relationships and dynamics on protein node abundance. Focusing on the epidermal growth factor receptor (EGFR) signaling network in HEK293T cells, we analyze 20 signaling proteins during a 1-h EGF stimulation time course using a panel of 35 antibodies. Data analysis with BP-R², a measure that quantifies complex signaling relationships, reveals abundance-de

SUBMITTER: Lun XK 

PROVIDER: S-EPMC5617104 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature