Analysis of chromatin accessibility in human epidermis identifies putative barrier dysfunction-sensing enhancers.

ABSTRACT: To identify putative gene regulatory regions that respond to epidermal injury, we mapped chromatin dynamics in a stratified human epidermis during barrier maturation and disruption. Engineered skin substitutes (ESS) cultured at the air-liquid interface were used as a model of developing human epidermis with incomplete barrier formation. The epidermal barrier stabilized following engraftment onto immunocompromised mice, and was compromised again upon injury. Modified formaldehyde-assisted isolation of regulatory elements (FAIRE) was used to identify accessible genomic regions characteristic of monolayer keratinocytes, ESS in vitro, grafted ESS, and tape-stripped ESS graft. We mapped differentiation- and maturation-associated changes in transcription factor binding sites enriched at each sta

SUBMITTER: Lander JM 

PROVIDER: S-EPMC5617145 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature